Plasmid
Part:BBa_K4514052:Design
Designed by: Daria Perminova Group: iGEM22_MiamiU (2022-10-12)
CRISPR-Cas13a expression vector
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 207
Illegal XbaI site found at 236
Illegal XbaI site found at 3088
Illegal XbaI site found at 3172
Illegal SpeI site found at 4045
Illegal SpeI site found at 7135
Illegal PstI site found at 6289
Illegal PstI site found at 7884 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 207
Illegal SpeI site found at 4045
Illegal SpeI site found at 7135
Illegal PstI site found at 6289
Illegal PstI site found at 7884
Illegal NotI site found at 6307 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 207
Illegal BglII site found at 22
Illegal BamHI site found at 1275 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 207
Illegal XbaI site found at 236
Illegal XbaI site found at 3088
Illegal XbaI site found at 3172
Illegal SpeI site found at 4045
Illegal SpeI site found at 7135
Illegal PstI site found at 6289
Illegal PstI site found at 7884 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 207
Illegal XbaI site found at 236
Illegal XbaI site found at 3088
Illegal XbaI site found at 3172
Illegal SpeI site found at 4045
Illegal SpeI site found at 7135
Illegal PstI site found at 6289
Illegal PstI site found at 7884
Illegal NgoMIV site found at 7433 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5980
Illegal BsaI.rc site found at 5968
Illegal SapI site found at 7373
Illegal SapI site found at 7583
Design Notes
N/A
Source
From (Kiga et al., 2020). Ordered off AddGene